Comparative of Phytochemical and Antimicrobial of Sesbania Grandiflora Leaves Extract

Abstract

The aim of this study was detection of the bioactive chemical constituents of sesban leaves extract by a qualitative analysis for each ethanol and methanol extract as used the Ethanol extract as antibacterial . The phytochemical analysis of the methanol and ethanol extracts of leaves of Sesbania sesban revealed the presence of flavonoids, alkaloids, steroids, phenols, carbohydrates and anthraquinones. While, they realed other chemical compound such as, tannins, saponins, terpenoids , amino acids and phytosterols. From the other hand, the ethanol extract of sesban was used as antibacterial against the gram positive bacteria (Streptococcus, Enterococcus faecalis (ATCC 29212), Staphylococcus aureus, (ATCC 25923),) and the gram negative bacteria (Escherichia coli (ATCC 25922), Salmonella typhi (MTCC 733), Proteus vulgaris (MTCC 1771), Pseudomonas aeruginosa (MTCC 424), Klebsiella pneumoniae (ATCC 15380) and was compared with standard drugs such as, gentomaicine and pencillin. The result showed greater inhibition zone in Pseudomonas (18 mm) at 1000mg/ml while, smaller inhibition zone in Streptococcus (10mm) at 500mg/ml.