Detection of HCMV DNA in Chronic Periodontitis Patients by Real time PCR and its relation with the severity of disease


Abstract:Chronic periodontitis is an inflammatory disease that extends into the tissuessupporting the teeth. Recent studies have demonstrated that various human herpes virusesespecially human cytomegalovirus (HCMV) may play a part in the pathogenesis of humanchronic periodontitis. The aim of this study is to detect HCMV DNA in saliva of chronicperiodontitis patients by real time polymerase chain reaction (PCR) test, measure its copynumber and find its relation with severity of the disease. The study sample consisted of fifty –three chronic periodontitis patients of both genders (27 males and 26 females) with agesranged from 25 to 45 years and all the selected patients have not any systemic diseases.Periodontal parameters used in this study were [plaque index (PLI), gingival index(GI), bleeding on probing (BOP), probing pocket depth (PPD) and clinical attachment level(CAL)]. Unstimulated saliva samples were collected from all subjects and examined by realtime PCR test for HCMV DNA detection. The results of this study observed that thepercentage of study group who were positive for HCMV DNA was (22.6%). The presentstudy observed that there was no relation between PLI and GI with HCMV DNA salivarylevel. Concerning BOP and PPD there was no relation found between BOP and PPD withHCMV DNA level. For CAL there was a significant relation found between CAL and HCMV