The Adhesion of Candida albicans to Epithelial Cells in Diabetes Mellitus Patients

Abstract

We determined the adhesion of Candida albicans to oral epithelial cells in diabetic patients by an autologous adhesion assay with exfoliated buccal or palatal epithelial cells and one strain of C. albicans isolated from each patient by concentrated oral rinse technique, and investigated the influence of the carbon source of the growth medium, source of epithelial cells, and the influence of gander and smoking on the adhesion. Glucose, sucrose and galactose were used as the predominant carbon source of the growth medium. The autologous strain of C. albicans adhered selectively to the oral mucosa of diabetic patients. Palatal epithelial cells retained significantly more C. albicans in vitro and adhesion influenced by the availability of sugars in the growth medium.