The Role of Antibiotic-Killed Staphylococcus aureus and Its DNA to Cause Arthritis in Rats


The purpose of this study is to investigate the capability of bacterial DNA compared to bacterial lysate in stimulating arthritis using rat model. One hundred mid-stream urine specimens were collected during November 2012 to January 2013, from patients suffering from urinary tract infections attending hospitals in Baghdad, Iraq. Susceptibility of Staphylococcus aureus isolates to antibiotics was examined. Twenty five isolates were identified as S. aureus and they developed multi drug resistance. S. aureus S1 lyaste and its DNA were intra-articulary injected in rats. The levels of IL-6, anti-ds DNA Ab and leukocytes count were measured. In general, IL-6, anti-ds DNA Ab and leukocytes count were significantly higher in sera of rats injected with cell lysate. Moreover, time period significantly (P< 0.05) affected the immunological aspects and joints damage. In a conclusion, bacterial lysate caused more damage to rat joints than DNA. Furthermore, bacterial lysate evoked immunological parameters more than DNA did.