Brucella melitensis Rev.1 live attenuated Vaccine and its DNA induced IFN-γ and anti-ds DNA antibodies production in rats


Brucellosis is one of the five common bacterial zoonoses in the world caused by organisms belonging to the genus Brucella. Immune recognition of bacterial infection may contribute to cytokine, as well as antibody production that are characteristic of innate and adaptive responses. In this study, the presence of attenuated live Brucella melitenses Rev1 bacteria or its DNA induced the immune system to produce IFN-γ and anti-ds DNA antibody. In respect to IFN-γ released, the B. melitensis Rev 1 attenuated live vaccine was able to stimulate the immune system more than the DNA (P≤0.05). Such finding could be attributed to the whole attenuated bacteria that have immunogenic factors other than the DNA like cell wall component and outer membrane. On the other hand, the B. meliensis Rev 1 DNA activated the B cell to secret anti-ds DNA antibodies significantly higher (P≤ 0.05) than live attenuated vaccine, and the level of antibodies was increased to parallel the concentration increases of injected DNA.