Evaluation of Anti-Helicobacter pylori Antibodies in A group of Iraqi Patients with Atherosclerosis and Coronary Artery Disease


Coronary artery disease (CAD) is the end result of the accumulation of atheromatous plaque within the walls of the coronary arteries resulting in decrease of oxygen and ischemic heart disease (IHD). It was consider as one of the most common diseases and major causes of morbidity and mortality worldwide. The present study evaluated the anti- Helicobacter pylori IgG and the role of virulence factor of H. pylori cytotoxin associated gene (Cag A) as a risk factors for CAD. Also the detection of proinflammatory markers such as C-reactive protein. The level of serum IgG was done by indirect immunofluorescent (IIF) whereas Cag A and high sensitive C- reactive protein (hs-CRP) measured by enzyme linked immunosorbent assay (ELISA). Seventy Iraqi patients with CAD were included in this study Their mean ages were 58.80 ± 5.13 years ranged between 41-85 years ; and 20 individuals as a control group which was divided into 2 subgroups: 10 apparently healthy volunteers (negative control) and the other subgroup contained 10 with normal coronary artery but had other heart disease except CAD (positive control). All blood samples were investigated biochemically such as glucose test, urea test , creatinine test and lipid profile test(cholesterol, triglyceride ,LDL and HDL) and all of them showed normal values. The result showed, there were a high significant differences (P˂ 0.01) of anti- H. pylori IgG between CAD patients and both positive and negative controls. 78.57% (55/70), 100% (10/10) and 0% (0/10) respectively. Also there were a significant differences (P˂ 0.05) in the mean value of Cag A antigen when compared to the positive and negative controls groups (2.74±0.19), (2.72±0.31) and (1.64± 0.16) respectively. Furthermore; the present study revealed significant differences of concentration levels of hs-CRP in patient group (4.95 ± 0.38 µg/ml) as compared to positive and negative control groups (0.77 ± 0.06 µg/ml, 3.96 ± 0.96 µg/ml). The results suggest there was a relationship between Helicobacter pylori infection and coronary artery disease.