TY - JOUR ID - TI - Quantitative assessment of Mutans Streptococci adhesion to coated and uncoated orthodontic archwires (In vitro study) AU - Aliaa Abdul Rhman Al-Lami علياء عبد الرحمن اللامي AU - Iman I. Al-Sheakli ايمان الشيخلي PY - 2014 VL - 26 IS - 4 SP - 156 EP - 162 JO - Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد SN - 18171869 23115270 AB - Background: The development of orthodontic biomaterials that attract less biofilm has been a goal for decades.Adhesion and colonization of cariogenic streptococci are considered to play key roles in the development ofenamel demineralization related to orthodontic materials. The aim of this study was to quantitatively evaluate theMutans streptococci adhesion to coated orthodontic archwires (Epoxy and Teflon) and uncoated archwires(stainless steel and nickel-titanium) with respect to incubation time in the presence and absence of saliva.Material and Method: Six types of archwires stainless steel and nickel titanium with two type of coating (Epoxy, Teflon)were used in this study. Twelve specimens of each archwire were incubated in sterilized unstimulated whole saliva(for the study group) and phosphate-buffered saline (for control group) for 2 hours, then incubated with suspensionof Mutans streptococci allowed to adhere for (5,90,180 minutes). Adhesion was quantitated by a microbial culturetechnique by treating the archwires with adhering bacteria with trypsin and enumerating the colony forming unit(CFU) counts of bacteria recovered after cultivation by using Dentocult SM kit.Results: There was significant difference among the tested archwire types in each time interval with the highestbacterial adhesion on the NiTi archwires in the absence of saliva. In the presence of saliva, the results revealed nonsignificantdifference at 5 min. while there was significant difference at 90 min and highly significant difference at 180min.Conclusion: The adherence of Mutans streptococci was decreased in the presence of saliva on different archwiresand the extended incubation time was significantly related to increase colony forming unit of Mutans Streptococci

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