TY - JOUR ID - TI - Identification the Effect of Some Antibiotics on the Activities of Brucella melitensis that Isolated from Patients in Baghdad تحدید تأثیر بعض المضادات الحیویة على فعالیة البروسیلا المالطیة المعزولة من المرضى المصابین في بغداد AU - Sumayah Abdul-Hussein Ibraheem سمیة عبدالحسین إبراھیم PY - 2015 VL - 15 IS - 1 SP - 1 EP - 5 JO - Al Mustansiriyah Journal for Pharmaceutical Sciences مجلة المستنصرية للعلوم الصيدلانية SN - 18150993 2959183x AB - Brucellosis constitutes a major health problem around the world, especially in developing countries. The sensitivity pattern of the Brucella isolates encountered in the most countries, numerous studies were indicated the sensitivity of Brucella melitensis to some antibiotics and resistance to another. The aim of this study to evaluated, in vitro, the effect of single and combination of some antibiotics on the activities of Brucella melitensis. This study included collection of (100) clinical sample of blood from different hospital in Baghdad. Six isolates of Brucella melitensis were identified and determine according to their biotypes. The susceptibility to different antibiotics was evaluated by disk diffusion method and MICs for some antibiotics used for brucellosis treatment were applied. Some antibiotics combination affect at concentrations of sub MIC on the growth of Brucella melitensis. Results showed that isolates of Brucella melitensi were sensitive completely to gentamycin, kanamycin and naldixic acid while it’s resistant to lincomycin, rifampicin, ciprofloxacin, Levofloxacin and doxycyline.The MIC to doxycycline and rifampicin were (40 and 5) μg/ml. respectively. There was an important effect on bacterial growth when the following antibiotics were mixed as (doxycyline+ rifampicin). The doxycycline changed the colonies from growth phase )pathogen phase( to intermediate phase while the rifampicin had no effect on colonies. The mixed of doxycycline with rifampicin have effect that change the pathogen phase to non pathogen phase (no growth phase). By this study, we can conclude a combination of antibiotics should be used to treat Brucellosis, tetracyclines, quinolones, trimethoprim/sulfamethoxazole, rifampicin, and streptomycin are commonly used preparations for this treatment.Key words: Brucella, MIC and Susceptibility.

تسبب البروسلا مشكلة صحیة كبیرة حول العالم وخاصة في الدول النامیة. درست حساسیة عزلات البروسیلا في معظم الأقطار و دلت الدراسات على حساسیة البروسیلا تجاه بعض المضادات الحیویة ومقاومتھا للبعض الاخر.تھدف ھذه الدراسة الى تقیم فاعلیة بعض المضادات الحیویة بشكل مفرد أو خلیط على فاعلیة بكتریا البروسیلا داخل المختبر.( شملت ھذا الدراسة جمع ( 100 ) عینة سریریة من دم المرضى من مستشفیات مختلفة في بغداد. تم الحصول على ( 6عزلات من إحدى أنواع بكتریا البروسیلا وتحدید أنماطھا الحیویة وھي البروسیلا المالطیة المعزولة من الانسان وتحدید ER -