TY - JOUR ID - TI - Evaluation of antibacterial effect of irrigant solutions (titanium tetra fluoride, green tea, sodium hypochlorite, normal saline) using real-time quantitative – polymerase chain reaction AU - Heba N. Yassin AU - Zeyneb A. A. Al-Dahan AU - Mohammed A. Hamod PY - 2015 VL - 27 IS - 3 SP - 140 EP - 145 JO - Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد SN - 18171869 23115270 AB - Background: Removal of bacteria from the pulp system by instrumentation of an infected root canal, will besignificantly reduced the number of bacteria, but it is well documented that instrumentation alone can-not cleanand kill all bacteria found on the root canal walls. Antibacterial irrigants are needed to kill the remainingmicroorganisms. The aims of this study was to assess antibacterial effect of titanium tetrafluoride (TiF4) solution andbrewing green tea against root canal bacteria and to compare with sodium hypochlorite and normal saline throughmicrobiological and molecular studies.Materials and methods: Microbiological study was carried out to determine the concentration of titaniumtetrafluoride and brewing green tea at which they exert antibacterial effect against ten swabs that had been takenfrom necrotic root canals that were incubated aerobically and anaerobically by paper disk diffusion test,whilemolecular study carried out among Forty children in which the antibacterial effect of titanium tetrafluoride andbrewing green tea compared with sodium hypochlorite and normal saline were assessed by real time polymerasechain reaction using SYBR Green.Results: The microbiological study results showed that TiF4 achieved maximum antibacterial effect at concentration5% against aerobic and anaerobic bacteria while green tea exhibited antibacterial effect when brewed for 20minutes at concentration 100mg/1ml against staphylococcus aureus, but not active against other microorganismslike Escherichia coli and streptococcus. While results of molecular study illustrated that sodium hypochlorite remainedthe most effective endodontic irrigant solutions followed by titanium tetrafluoride then green tea while normal salineshowed no antibacterial effect. Statistically titanium tetrafluoride, green tea and sodium hypochlorite havesignificant differences compared to normal saline.Conclusions: This study revealed that, titanium tetra fluoride and brewing green tea can be used as antibacterialirrigant solutions for root canal treatment in children

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