TY - JOUR ID - TI - In-Vitro evaluation of Isolated Staphylococcal-bacteriophage in Killing Methicillin-Resistant Staphylococcus aureus AU - Sarhan Rashid Sarhan AU - Orooba Mohammed Saeed Ibrahim AU - Serwa Ibrahim Salih PY - 2016 VL - 7 IS - 2 SP - 218 EP - 240 JO - Kufa Journal For Veterinary Medical Sciences مجلة الكوفة للعلوم الطبية البيطرية SN - 20779798 29598478 AB - The present study was carried out to isolate and identified the Staphylococcal-specific bacteriophage from swage water by using agar overlay method and to investigate the anti-Staphylococcal activity of هsolated Bacteriophage in-vitro. Two experiments were performed in this present study; the first one was isolation and identification of Methicillin-Resistant Staphylococcus aureus (MRSA) from post-traumatic bone infection. While the second experiment included study the infectivity of bacteriophage in-vitro to MRSA as well as effect of temperature and pH on the infectivity of phage was also studied. Results of antimicrobial susceptibility test of S. aureus isolates revealed that most of isolates were resistant to Methicillin, Cefoxitin and Pencillin G. Staphylococcus aureus phage was isolated from sewage samples by agar overlay method, the phage was characterized by clear, circular plaques ranged between 2-3 mm in diameter. TTA method for determined the Phage MIC appears to be both reliable, and reproducible.

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