TY - JOUR ID - TI - Molecular Detection of Virulence Factors Genes in Pseudomonas aeruginosa Isolated from Different Infections Cases in AlDiwaniyaHospital AU - Wafaa A. J. Al-Kaaby PY - 2015 VL - 2 IS - 20 SP - 53 EP - 58 JO - Al-Qadisiyah Journal of Pure Science مجلة القادسية للعلوم الصرفة SN - 19972490 24113514 AB - Pseudomonasaeruginosa is the most important and dangerous organism in humaninfections due to production of several extracellular and cell-associated virulence factors thatcause severe tissue damage, the most important virulence factors including (exoenzyme S,exotoxinA and lipoprotein), these factors was encoded by (exoS, toxA and oprIgenerespectively). In this study we used polymerase chain traction technique (PCR) for detectionvirulence factor genes producing by P.aeruginosa that isolated from wound, burn andpulmonary tract infections patient swab samples. The PCR results was show that all P.aeruginosa isolates was carried virulence factors genes with difference in prevalence betweenthem. The burn infection P. areuginosa isolates were show high prevalence of virulencefactors genes more than wound and pulmonary tract infections isolates as well as thevirulence factor gene (ToxA) was show high production in most isolates. In this study weconcluded that the production of virulence factors genes in P. aeruginosa is important tohuman infection especially (ToxA) gene and the PCR technique is very specific and fastmethod in detection virulence factor genes in P. areuginosa .

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