TY - JOUR ID - TI - EVALUATION OF MEMBRANE INTEGRITY OF BULL FROZENTHAWED SPERM USING WATER AND HYPO OSMOTIC SWELLING TEST AU - Godratollah Mohammadi, AU - Hamed Mahdion PY - 2017 VL - 16 IS - 2 SP - 131 EP - 143 JO - Basrah Journal of Veterinary Research مجلة البصرة للابحاث البيطرية SN - 18138497 24108456 AB - Assessment of the sperm membrane functional status appears to be a significant markerfor the fertilizing capacity of spermatozoa. The hypo osmotic swelling test (HOST) is one ofthe best methods to evaluate sperm membrane integrity. In the current study, we used DWand hypo osmotic solutions of 50 and 100mOsm/l of dextrose/NaCl, NaCl, sucrose andfructose. Based on the results, Among the dextrose/NaCl, NaCl, sucrose, and fructosesolutions and DW, Maximum numbers of swollen of bull frozen-thawed spermatozoa wereobserved with DW and dextrose/NaCl solution at 50 mOsm with average response by61.20±8.677 and 47.90±10.181 respectively. The HOST response at 3 and 60 min for all ofsolutions were positively correlated to each other and there was no significant differencebetween the responses to the HOST at 3 and 60 min after incubation in all of solutions. Thesignificant correlation was observed between motility and dextrose/NaCl at 50 mOsm,sucrose 50 and 100 mOsm, NaCl 50 mOsm and DW. The high relationship was betweenmotility and DW and dextrose/NaCl at 50 mosm. There was no significant correlationbetween DW and all of hypoosmotic solutions with staining of the spermatozoa byeosin/nigrosin. In conclusion, the water test can be efficiently used for the evaluation of thefunctional integrity of the plasma membrane of bull frozen-thawed spermatozoa. Thehypoosmular solution of dextrose/NaCl at 50 mOsm is a good medium to evaluate bull frozenspermatozoa. The used of HOST and motility are better tests to evaluate bull frozen thawedsperm than eosin-nigrosin. The short HOST procedure (3 min) is suitable method forevaluating of membrane integrity of bull frozen/thawed spermatozoa

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