TY - JOUR ID - TI - 8-MULTIPLEX PCR DETECTION OF ERYTHROMYCIN RESISTANCE GENES IN COAGULASE NEGATIVE STAPHYLOCOCCI ISOLATED FROM COWS IN BASRAH, IRAQ AU - Jean M. Mezban, Mohammed H. Khudor , Basil A. Abbas PY - 2018 VL - 17 IS - 1 SP - 86 EP - 102 JO - Basrah Journal of Veterinary Research مجلة البصرة للابحاث البيطرية SN - 18138497 24108456 AB - This study was conducted to identify the erythromycin resistance genes in thecoagulase-negative staphylococci (CoNS) and its molecular characterization afterisolating the bacteria from the samples of domestic animals and their products during theperiod from September 2016 to March 2017 from different areas in Basra city. 200samples were collected from animals including: 40 samples from meat, 50 samples fromraw milk, 30 samples from treated milk, 40 samples from cow's nasal swabs and 40samples from cow's teat swabs. Results showed that from 200 collected samples only 108were CoNS distributed in 22(15.1%), 18(12.4%),10(6.8%), 26(17.9%),32(22%), frommeat, raw milk, treated milk, nasal swabs and teat swabs respectively. Samples wereplanted on the selecting mannitol salt medium to isolate Staphylococcus spp. which hadthe ability to grow on the mentioned medium. When the coagulation test was performed,some isolates were not able to produce the coagulation enzyme, and the results showedthat 108 isolates were coagulase negative (54%). Twenty-two isolates of minced meat55%, 18 isolates of cow's milk (36%), 10 isolates of milk sold (33%), 26 isolates of thenose of the animal (65%) and 32 isolates of animal teat swabs (80%). Twenty-fiveisolates of these negative staphylococci were identified using VITEK 2 kit. The resultshowed that 10(40%) isolates identified as coagulase negative Staphylococcus and fall in87four species including 4 (40%) Staphylococcus lentus, 4 (40%) Staphylococcusgallinarum, 1 (10%) Staphylococcus haemolyticus, and 1 (10%) Staphylococcuschromogen. When the ermA, ermB, ermC and msrA genes were investigated by PCR theresult showed that they contain the genes in a percentage 5%, 20%, 20% and 5%respectively. By using the multiplex PCR molecular weight technique (ermA, ermC) and(msrA, ermC), the ratio of both genes was 15% and 5% respectively.

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