TY - JOUR ID - TI - Identification of Cryptococcus neoformans Isolates by PCR-ITS regions AU - Israa A. Al-Temimay AU - Esraa Muslim Aswad PY - 2018 VL - 59 IS - 4A SP - 1792 EP - 1805 JO - Iraqi Journal of Science المجلة العراقية للعلوم SN - 00672904 23121637 AB - The aim of the study was molecular detection of C. neoformans that isolated from 150 (88 female and 62 male) clinical samples (sputum samples) from pulmonary patients in Baghdad. The diagnoses of Cryptococcus neoformans in samples was done by using direct microscopic examination, culture media and PCR Technology. Microscopic examination and cultured revealed that 65 out of 150 (43.33 %) samples were positive and the others samples were Negative. Results of the genetic diagnosis looking for the fungi causing cryptococcosis using primers specific for ITS gene which were specially designed for this study revealed that 6 (4 %) of sputum samples were positive. In this study used the PCR technology due to the present of high specificity and sensitivity for the diagnosis of C. neoformans added to that which is cheaper and faster than the conventional methods currently used in hospital and laboratories.

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