TY - JOUR ID - TI - Estimation of zeta‑chain‑associated protein 70, interleukin‑6 and interleukin‑10 levels in sera of Iraqi newly diagnosed chronic lymphocytic leukemia AU - Hassnien S. Muslit AU - Alaa Fadhil Alwan AU - Haider S. Al‑Dahery PY - 2016 VL - 5 IS - 2 SP - 173 EP - 177 JO - Iraq Joural of Hematology المجلة العراقية لامراض الدم SN - 20728069 25432702 AB - BACKGROUND: Chronic lymphocytic leukemia (CLL) is the most common type of leukemia in the Western world.The zeta‑chain‑associated protein (ZAP‑70) an intracellular tyrosine kinase which play an important role in T‑cellreceptor signaling, natural killer cell activation, and early B‑cell development. Interleukin‑10 (IL‑10) productionhas strong immunosuppressive effects through inhibition of Th1 type cytokines. IL‑6 is a pleiotropic cytokineproduced by a variety of cell types, including normal hematopoietic cells, and lymphocytes.OBJECTIVES: The objective of this study is to assess the level of ZAP‑70, IL‑10, and IL‑6 in CLL and to correlatethese levels with prognosis.MATERIALS AND METHODS: A prospective cohort study carried out at the National Center of Hematology fromOctober 2013 to September 2015. Eighteen patients with newly diagnosis of CLL compared to 19 apparentlyhealthy controls were also involved in this study. ZAP‑70 was measured by while IL‑10 and IL‑6 were measuredusing serological methods including the enzyme‑linked immunosorbent assay.RESULTS: ZAP‑70 range between (24% and 90%) with a mean of 46.89 ± 19.15, two patients out of 18 werenegative (<24%) for ZAP‑70 as compared with control. The levels of IL‑6 in the serum of untreated patients withCLL were increased in compared with healthy control (2.53 ± 1.98), ranged between (0.12 and 6.94) pg/ml. Therange of the IL‑10 expression of the untreated CLL patients was between (the lowest positive value (212 pg/ml)and which is the highest positive value (987 pg/ml) with a mean 614 ± 301 pg/ml, with all the morphologicallydiagnosed CLL cases show positive expression for IL‑10.CONCLUSION: ZAP‑70 level was higher in CLL patients than control and immunochemotherapy can normalizethis which indicated good response to treatment. On the other hand, IL6 and IL10 were also higher in patientswith CLL but not affected by therapy.

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