TY - JOUR ID - TI - Comparing the Activation Role of Centrifugation Swim-up and the Density Gradient Combined with ALLGrad Sperm Preparation Techniques AU - Sahar Muhssin Muhammad AU - Muhammad-Baqir Muhammad-Rashad Fakhrildin PY - 2019 VL - 9 IS - 1 SP - 111 EP - 134 JO - Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم SN - 26166984 22180265 AB - In about 40% of infertile couples, the husband was found to be either the single or a contributing cause of infertility and the systemic management ready for those couples with male infertility are restricted. 135 couples with infertility shared herein; the husbands required in vitro activation for their sperm samples, their wives however; had no fertility problem and were submitted ovarian induction protocol. However; Sperm preparation was done using both, the centrifugation indirect swim-up technique with Global medium and density gradient with ALLGrad solution. While ALLGrad 45% is a ready-to-be-used upper layer. There was an acceleration in seminal parameters after preparation using both various techniques. So, a highly significant increment (P≤0.001) in the percentages of progressive forward motility was in grades (A and B) and grade (A) progressive sperm motility, also a significant enhancement (P≤0.05) in percentage of morphologically normal sperms when applying density gradient mechanism in relation to centrifugation swim-up technique. This study concludes that the use of density gradient mechanism with ALLGrad solution and Global culture medium are more efficient in achieving better percentages of progressive forward motility grades (A and B), grade (A) progressive motility and morphologically normal values of spermatozoa than centrifugation indirect swim-up technique by using Global culture medium. However, it was found there was a non-significant difference when we use both techniques concerning other sperm parameters.

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