@Article{, title={Impact of Low- Energy Fluencies Laser Irradiation at 532 nm on Neutrophils and Lymphocytes Blood Counting /}, author={Zahra, J.M.A. Al-Timimi /}, journal={Iraqi Laser Scientists Journal مجلة علماء الليزر العراقية}, volume={1}, number={3}, pages={21-28}, year={2019}, abstract={Lymphocytes are a kind of WBCs that are parts of the subordinate immune defense tools, they are performing to identify antigens, and produce antibodies destroy cells, which induce damage. Neutrophils are one form of granulocytes containing granules of enzymes in their cytoplasm which; are released during the infections and allergic reactions Low-level laser radiation has various impacts on the cultured human cells in vitro; despite a little is known on such rules in the whole blood. The current research considered to prove that the low energy laser intensity, may have a significant or counteractive influence on Lymphocyte and Neutrophils counts in human whole blood in vitro. The influence of different laser energies on the blood samples that are taken from adult patients using low-dose laser irradiating was been investigated. The samples of blood were collected from 100 adult patients , 5 ml each divided into 2.5 for each irradiation and control (non-irradiated) samples and anti-coagulant. Then, samples were separated into four groups, for irradiation; with different laser doses. In the 1st. group, the laser intensity was (0.5 Joule /cm2) at an exposure duration of 1 Sec followed by (1.5 Joule /cm2, 3 Joule /cm2 and 5 Joule /cm2) for the 2nd., 3d. and 4th. groups at the time of (3, 6, 10 Sec) respectively, 532 nm laser was used as a radiation source. An Automated Hematology Analyzer (Sysmex XE -2100™) was utilized to estimate the values of irradiated & non-irradiated. The outcomes note that the 532nm laser light on whole blood samples did not produce any meaningful changes or effects in the count of Neutrophils and Lymphocytes. Lymphocytes count remained unchanged for all the experimental groups. In our perception of the neutrophils interchanges with the laser inferences, we remarked that there is a small variation between irradiated and control samples within the 1st group; which appear that there was a smallest drop in the count after laser. This may relate to the high differentiation in these cells. In conclusion, Our outcomes obviously demonstrate that Neutrophils and Lymphocytes cells counts are not altered by 532nm laser irradiation, which ultimately strengthens the hypothesis that one mechanism for the bio - stimulation impact after laser irradiation controls is the reaction of light with the hemoglobin, contributing in oxygen radical production.

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