TY - JOUR ID - TI - Estimation of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Antimicrobial peptides of Saccharomyces boulardii against Selected Pathogenic Strains AU - Venkateswarulu T.C PY - 2019 VL - 5 IS - 4 SP - 266 EP - 269 JO - Karbala International Journal of Modern Science مجلة كربلاء العالمية للعلوم الحديثة SN - 2405609X 24056103 AB - Saccharomyces boulardiiis used for antimicrobial peptide production and so can be termed as bacteriocin molecules with probiotic potential. Antimicrobial peptides are extracted from the culture broth and followed the purification using Sephadex G-50 column. Further, the inhibition activity was determined against selected microorganisms and inhibition zone against streptococcus pneumoniaewas found to be highest among selected microbes. The fourth fraction of the peptide after purification was shown a 26 mm bacterial inhibition zone determined by agar well diffusion method. SDS-PAGE analysis revealed the protein band corresponding to 18 kDa was observed. The MBC/MIC study proved that the peptide extract of S. boulardiihas bactericidal activity.

ER -