@Article{, title={Antimicrobial sensitivity, extended spectrum beta-lactamase (ESBL)production and plasmid profile by microorganisms from Otitismedia patients in Owo and Akure, Ondo State, Nigeria}, author={A.K. Onifade, C.O. Afolayan, O.I. Afolami*}, journal={Karbala International Journal of Modern Science مجلة كربلاء العالمية للعلوم الحديثة}, volume={4}, number={3}, pages={332-340}, year={2018}, abstract={Microbiological analyses were carried out on clinical samples from Otitis media patients attending two governmenthospitals in Akure and Owo, Ondo State, Nigeria. Bacteria isolates such asEscherichia coli,Klebsiella pneumoniae,Proteusmirabilis,Pseudomonas aeruginosa,Staphylococcus aureus,Streptococcus pneumoniae,Staphylococcus epidermidis,Strep-tococcus pyogenesand fungal isolates such asAspergillus niger,Aspergillus fumigatus,Aspergillus flavusandCandidaalbicanswere identified from the analyzed samples. All the bacteria isolates were screened for beta lactamase production ofwhich varying percentages of the characterized isolates were positive for beta-lactamase production such asE. coli(12.1%),K. pneumoniae(16.7%),P. mirabilis(8.2%),P. aeruginosa(28.8%),S. aureus(11.1%),Strep. pneumoniae(13.3%),S.epidermidis(7.1%) andStrep. pyogenes(10.7%), respectively. The results obtained from antibiotic sensitivity revealed thepresence of multiple antibiotic resistant isolates (MDRIs) present among the bacteria isolates obtained and that Nitrofurantoinand Ofloxacin were the most effective antibiotics against the bacteria isolates tested. The Multiple Drug Resistant Isolateswere subjected to plasmid profile analysis and they were cured of their resistant factor plasmids; they were subjected to post-plasmid curing antibiotic resistant assay where it was discovered that their initial antibiotic resistance was plasmid-mediated.Furthermore, the antifungal sensitivity test of fungal isolates revealed that Fluconazole and Clotrimazole were the mosteffective antifungal agents against the fungal isolates obtained from the samples analyzed.

} }