TY - JOUR ID - TI - Association between exposure to nitric oxide and changes in selectmolecular markers of health among men in the gold jewelrymanufacturing industry AU - Meyyazhagan Aruna,*, Balasubramanian Balamuralikrishnana,Alagamuthu Karthick Kumara, Shanmugam Sureshkumara,Shafiahammedkhan Mustaqahameda, Subramanium Mohanadevia,Murugesh Easwaranc, Nitya Ramand, Kuchi Bhotla Haripriyaa,Hemalatha Palanivele, Vellingiri PY - 2016 VL - 2 IS - 3 SP - 178 EP - 187 JO - Karbala International Journal of Modern Science مجلة كربلاء العالمية للعلوم الحديثة SN - 2405609X 24056103 AB - Gold jewelry workers are occupationally exposed to nitric oxide (NO) during the purification of gold. The present studyinvolves the evaluations of chromosome aberrations (CA), micronucleus (MN) and comet assay in lymphocytes and identifies theinfluence of polymorphisms in DNA repair genes (XRCC1280Arg/His,XRCC1399Arg/Gln) among the gold jewelry workersoccupationally exposed to nitric oxide (NO). Among the 84 samples undertaken for study, the non-exposed (categorized basedonly on age being group I<35years; group II35 years) and exposed subjects (categorized based on age and work duration)constituted 42 subjects each. With increase duration of years of exposure, an increased level of chromosomal damage wasobserved. Likewise, a higher degree of chromosomal damage and MN was observed in exposed subjects of group II with a totalCA of 8.07±3.29 and MN of 10.61±2.25 when compared to group I. Significant increases were also indicated with the overallCA frequency for the exposed subjects for chromatid, chromosome type aberrations and the MN/1000 binucleated cells inperipheral lymphocytes (p<0.05) in comparison with non-exposed. The analysis of mean tail length of comet assay shows theDNA damage among the group II exposed subjects exhibits higher degree of chromosomal damage, indicating chronic exposureof NO causes the genetic modification, largely influenced by the polymorphic genotypesXRCC1280Arg/His (p<0.001) andXRCC1399Arg/Gln (0.524). In conclusion, this result of work provides evidence for an apparent genotoxic effect associated with NO exposure. Our result reinforces the higher sensitivity of cytogenetic assays for the biomonitoring of occupationally exposedpopulations. So there is a strong need to educate those who workwith potentially hazardous heavy metals about their adverseeffects and highlight the importance of using protective measures.

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