TY - JOUR ID - TI - Induction of intrinsic apoptotic pathway in human breast cancer(MCF-7) cells through facile biosynthesized zinc oxide nanorods AU - Krishnamoorthy Kavithaaa, Manickam Paulpandib,*, Thondhi Ponrajb,Kadarkarai Muruganb, Sundaravadivelu Sumathia PY - 2016 VL - 2 IS - 1 SP - 46 EP - 55 JO - Karbala International Journal of Modern Science مجلة كربلاء العالمية للعلوم الحديثة SN - 2405609X 24056103 AB - Biosynthetic and environment friendly technology for the synthesis of zinc oxide (ZnO) are believed to be nontoxic, biosafe, andbiocompatible nature. It also has been used as drug carriers, cosmetics, and fillings in medical materials. The present work reportsthe induction of intrinsic apoptotic pathway in Human Breast Cancer (MCF-7) cells through biosynthesized Zinc Oxide Nanorods.The UVevisible spectrum showed an absorption peak at 375 nm that reflects surface Plasmon resonance (SPR) ZnONRs and alsoconfirms the particles stability. TEM photograph showed that the green synthesized ZnONRs were highly crystaline nature and rodlike structure with an average size of 100 nm. The Zeta potential value of13 mV revealed the surface charge of green synthesizedZnONRs. In this study, we found that the synthesized ZnONRs exhibited a dose-dependent cytotoxicity against MCF-7 cells. Theinhibitory concentration (IC50) was found to be 10mg/mL. An induction of apoptosis was evidenced by AO/EtBr apoptotic stainingmethod. Based on the FTIR spectral data,b-Santalol a kind of sesquiterpen present inSantalum albumleaf extract is responsible forreduction and stabilization of ZnONRs by this approach are quite stable and no visible changes were observed even after longperiod. These terpene substances could have acted as both reduction and stabilizing agents preventing the aggregation of particles.Thus, the nature of biosynthesis and the therapeutic potential of ZnONRs could prepare the way for further research on the design ofgreen synthesis therapeutic agents, particularly in nanomedicine, for the treatment of cancer.

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