TY - JOUR ID - TI - The protective role of ascorbic acid in the reproductive functions of female laboratory mice treated with methomyl pesticide AU - Haider H. Neamaa AU - Faris S. Kata PY - 2020 VL - 46 IS - 2 SP - 89 EP - 102 JO - Journal of Basrah Researches (Sciences) مجلة ابحاث البصرة ( العلميات) SN - 18172695 2411524X AB - The present study aimed to evaluate the preventive role of ascorbic acid in female laboratory mice treated with the methomyl Carbamate insecticide, The research included a study of physiological changes in the level of the sex hormone estrogen and the Phase Oogenesis and measuring of the antioxidant glutathione and catalase enzymes level . In the current study, 40 females mice of age (10-12 weeks) and weight (23-25 g) were used with sexual experience and were divided into five groups. Each group contained eight mice. The first group was injected with the physiological solution, The second group treated with low dose of the pesticide 1 mg / kg methomyl pesticide, the third group (with high dose of the pesticide) was injected with a concentration of 2mg / kg of methomyl pesticide, the fourth group was injected with a concentration of 200 mg/kg of ascorbic acid After being injected with the 1 mg / kg dose of the pesticide and the fifth group was injected with a concentration of 200 mg/kg of ascorbic acid after being injected with the 2 mg / kg dose of the pesticide. The results showed a significant decrease in the level of estrogen hormone in the serum of female laboratory mice with both doses treated with the pesticide. The injection of mice treated with ascorbic acid also improved the level of the hormone, which led to an increase in its level in the serum compared to its level in the two groups treated with the pesticide only (methomyl), as the statistical analysis showed that a reduction occurred significant number of Ovarian vesicles and the two doses treated with the pesticide, while treatment the mice with ascorbic acid recorded a distinct protective role in the process of Oogenesis. In addition, ascorbic acid preserved the levels of antioxidants in the serum of mice after it increased significantly due to treatment with methomyl only.

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