TY - JOUR ID - TI - Immunohistochemical Assessment of MGMT Expression in Human Gliomas. A Clinico-Pathological Study AU - Mustafa Hafudh Hamoodi, Ban J. Qasim PY - 2021 VL - 20 IS - 2 SP - 155 EP - 164 JO - Iraqi Postgraduate Medical Journal المجلة العراقية للاختصاصات الطبية SN - 83601608 98932708 AB - BACKGROUND:Gliomas are the most common primary malignant brain tumors in adults. They can occur anywherein the central nervous system but primarily occur in the brain and arise in the glial tissue. O6-alkylguanine DNA alkyl transferase is a protein (DNA repair molecule) encoded by the O6-methylguanine DNA methyltransferase (MGMT) gene in human, and are able to remove alkyladducts from the O6 position of guanine, and the O4 position of thymine, restoring these DNA basesand preventing temozolomide induced cell death.OBJECTIVE:To assess the immunohistochemical expression of MGMT in human glioma.METHOD:This retro and prospective study included 56 tissue paraffin blocks of intracranial gliomas assignedas cases group, and 28 tissue paraffin blocks of normal brain tissue as control group. From eachblock, two sections were taken; one was stained with the routine hematoxylin and eosin stain, andthe other was stained immunohistochemically for marker MGMT.RESULTS:MGMT showed a highly significant difference in its expression between the control group and thedisease group (p value<0.001). MGMT showed a highly significant relation between its expressionand the age of patients with glioma (p value< 0.001). MGMT expression showed no significantcorrelation with other clinicopathological parameters like the histological types and gender (pvalue<0.065 and 0.255) respectively.CONCLUSION:MGMT revealed a highly significant difference in its expression in brain tumors (gliomas)compared to control group which may reflects the need in proliferating cells for greater capacity torepair O6-alkylguanine adducts before replication. Besides there was a significant correlationbetween age and MGMT expression which may reflect processes associated with the physical andfunctional maturation of the CNS during life.

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