TY - JOUR ID - TI - Preparation of Modified Selective Medium for Isolation of Enterococcus faecalis in Pure Culture from Heavy Sources (Rapid Diagnosis) AU - Jawad Kadhim Tarrad AL-Khafaji PY - 2021 VL - 18 IS - 4 SP - 340 EP - 342 JO - Medical Journal of Babylon مجلة بابل الطبية SN - 1812156X 23126760 AB - Subject: This research focuses on the preparation of a new modified selective medium for the primary isolation of Enterococcus faecalisin pure culture from heavy and clinical sources. Materials and Methods: The modified selective medium was prepared and named asEnterococcus Jawad (EJ)–Medium. The new medium was prepared by adding 0.4 g of sodium azide, 2 mL of 0.1% crystal violet (CV),and 60 g of sodium chloride in a liter of Brain-Heart Infusion Agar (BHIA), and the pH of the medium was adjusted to 8.6. Theprimary isolation of E. faecalis from heavy bacterial sources and clinical infections was performed by culturing all collected sampleson EJ-medium and comparing them with other approved selective media, such as Azide-BHIA, CV-blood agar, and Enterococcusselective agar (ESA), to estimate the ability of these media in isolating E. faecalis (during primary isolation) in pure culture. Results:The results showed that the EJ-medium had a high rate of isolation, 100%, of E. faecalis in pure culture from heavy sources (humanfeces and sewage water) and from clinical sources (urine, bedsore, and blood) when compared with other culture media that werealready used for isolation of this organism. The Azide-BHIA and CV-blood agar showed no high purity in an initial isolation of theorganism. Conclusions: From these results, we concluded that the EJ-medium is highly efficient in the primary isolation of E. faecalisin pure culture by one step from different environmental and clinical sources; thus, it does not need to be diagnosed by classicalmethods that are expensive and consume a long time. Since Azide-BHIA, CV-blood agar, and ESA were not exactly selective mediafor the isolation of E. faecalis in pure cultures from heavy sources, their use was limited in clinical cases.

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