TY - JOUR ID - TI - PER β- lactamase in Klebsiella pneumoniae resistant to carbapenem from Al-Hillah Teaching Hospital environment PER β- lactamase in Klebsiella pneumoniae resistant to carbapenem from Al-Hillah Teaching Hospital environment AU - Fatima Moeen Abbas PY - 2021 VL - 9 IS - 3 SP - 128 EP - 132 JO - Journal of Genetic and Environmental Resources Conservation مجلة صيانة المصادر الوراثية والبيئية SN - 23068663 23068280 AB - The aim of this work is to identify the existence of K.pneumoniae recovered from various places of Al-Hillah Teaching Hospital environment / Hillah city/ Babylon Province and detect blaPER gene among carbapenem resistant isolates. One hundred samples were collected by swabbing various environmental locations between the period from October, 2017 and January, 2018.Klebsiella pneumoniae were detected in 13(13%) location. ESBL production was screened phenotypically using CHROMagar technique. Ten (77%) K.pneumoniae isolates with positive result. Disk diffusion method was used to test susceptibility of ESBL-K.pneumoniae producers .Higher prevalence rates of resistance were recorded for ampicillin (80%) and amoxicillin-clavulanic acid (70%) antibiotics. Two isolates were resistant to meropenem, these were checked for the existence of PERβ-lactamase using Polymerase Chain Reaction (PCR) technique. blaPER gene was detected in 1 (50%) selected isolate.

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