@Article{, title={Identification and study of cultural conditions affecting Neomycinantibiotic produced by Streptomyces sp. N دراسة الظروف الزرعية المؤثرة على انتاج المضاد الحيوي النيومايسين المنتج من قبل العزلة المحلية ستربتومايسين N}, author={Abd.l Malik W and Alkaragoli, R. S. and Algafari, R. N.}, journal={Tikrit Journal of Pharmaceutical Sciences مجلة تكريت للعلوم الصيدلانية}, volume={4}, number={1}, pages={37-43}, year={2008}, abstract={Cuftural conditions affecting antibiotic production by local isolate of Streptomyces sp. N were
studied. Cultural conditions included groMh medium, growth temperature,pH, and ion effect. The study
showed that this local isolate was able to produce neomycin antibiotic. This compound showed a
biological activity against gram - positive, and gram - negative bacteria. This effect was variable
depending on the factors studied. Media of antibiotic production, R2 medium, and complete medium
rvere better for Slreptomyces growth. Growth of the organism for 5 days gave the largest inhibition zones
against test bacteria. The organism shorved better gror,l,th and antibiotic production when cultivated at
370C. The pH of the medium showed a dramatic effect on antibiotic production since low pH had
increased antibiotic release to the medium resulting in observed increased in the biological activity. Ions
like phosplrate and citrate shorved diff'erenteff'ecton the antibiotic production. Tlrc phosphate had shorvn
an enltancing effect rvhere as citrate inhibit antibiotic production and lowercd the grolvth of the organism
on cultivation media.

} }