TY - JOUR ID - TI - Detection of Trypanosoma spp. in Dogs Using Different Laboratory Techniques in Mosul City, Iraq الكشف عن انواع المثقثيات في الكلاب تاستخدام تقنيات مختثرية مختلفة في مدينة الموصل- العراق AU - Mohammad A. S. Altaliby PY - 2022 VL - 21 IS - 1 SP - 38 EP - 48 JO - Basrah Journal of Veterinary Research مجلة البصرة للابحاث البيطرية SN - 18138497 24108456 AB - Canine trypanosomiasis is caused by a parasite of the genus trypanosome. It can be transmittedto different animals such as ruminants, horses, cats, in addition to human. Dogs play a crucialrole in transmission of trypanosome, as they are the primary domestic animals that contribute tothe parasite's transmission and maintenance cycles. Thus, the aim of this research was to estimatethe prevalence of trypanosomiasis in dogs in Mosul city, Iraq using routine laboratory techniquesand molecular approaches. The results of study showed that a total prevalence rates ofTrypanosome spp. in 85 dogs were 18.8%, 24.7%, 38.8%,7.05% using blood staining smear,microhaematocrit centrifugation technique(MHCT) and conventional PCR technique (ITS-1gene and RoTat 1.2 VSG gene) respectively. A significant differences in the prevalence ratebetween males (18%) and females (20% ) at P≤0.05, based on blood staining smear. While, nosignificant differences in the prevalence rate between males(24%, 36%, 16.6%) and females(25.7%, 42.8%, 20% ) at(P≤0.05), based on MHCT, conventional PCR (ITS-1gene and RoTat1.2 VSG gene) respectively. The results finding indicated presence of significant differencesamong different ages of dogs using microscopical and conventional PCR (P≤0.05), A dogs fromfour to five years old have the highest prevalence rate(68.7%) when compared with others, andTrypanosoma evansi was determined in old dogs(3-5 years) only. results revealed that a perfectagreement between blood staining smear method and MHCT, and substantial agreement betweenblood staining smear method and conventional PCR and moderate agreement between MHCTand conventional PCR. In conclusion the prevalence of trypanosome infection in dogs(stray andhoused) is very high in Mosul city, Iraq

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