TY - JOUR ID - TI - Improvement Efficacy of Bacillus Subtilis Cellulose Hydrolyzing by Using Cold Plasma Technique AU - Isam Shaker Hamza AU - Labeeb Ahmed Alzubaidi AU - Aziz Latif Jarallah AU - Rehab S. Ramadhan PY - 2022 VL - 63 IS - 4 SP - 1423 EP - 1430 JO - Iraqi Journal of Science المجلة العراقية للعلوم SN - 00672904 23121637 AB - Bacillus subtilis, an isolate of bacillus genus, was obtained from the laboratoriesof Ministry of Science and Technology. The best efficient Bacillus subtilis isolate incellulose and semi-cellulose hydrolysis was treated with Dielectric-barrier Discharge(DBD). Atmospheric cold plasma technique (non-thermal) was used by exposingthem at different times (2, 3, 4 and 5 mins) separately as a first stage, and then 60seconds after any treatment separately as a second stage. After 48 hours, thedifference between the plasma source and the sample was fixed at 0.5 cm. Theresults showed a variation in the growth of the isolate according to the exposure timeby the appearance of culture turbidity and the estimation of optical density. Positiveresults appeared between exposure times, the amount of optical density, and thecellulose and semi-cellulose decomposition into glucose. Bacillus subtilis increasedits efficacy in cellulosic hydrolysis and semi-cellulosic materials. Bacillus subtilisshowed malleability and the ability to increase the efficiency in cellulose and semicellulosematerials hydrolysis. We conducted a new and extensive study by usingcold plasma technique to

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