@Article{, title={C60 Hydrofullerene Induced Autophagy and Ameliorated GFAP In H2O2 Treated Human Malignant Glioblastoma U-373 Cell Line}, author={Aryan M. Faraj Medical Laboratory Science Department, Technical College of ApplieScience, Sulaimani Polytechnic University,, Aryan.fraj@spu.edu.iq Can A. Agca Molecular Biology and Genetics Department, Bingol University, Victor S. Nedzvetsky Molecular Bi}, journal={Karbala International Journal of Modern Science مجلة كربلاء العالمية للعلوم الحديثة}, volume={8}, number={3}, pages={486-492}, year={2022}, abstract={Glioblastoma is one of the most combative astrocytoma that is resistant to chemotherapy and radiotherapy. Thisresistance makes it very difficult to treat. However, researches have shown that nanoparticles especially C60 hydrofullerene have antioxidant and anticancer activity. The effect of C60 hydrofullerene in cancer has been extensivelystudied; however, the potential regulation of autophagy and modulation of the Glial Fibrillary Acidic Protein (GFAP)gene has not been addressed in glioblastomas. Glioblastoma U-373 cell was treated with 0.5 mM of C60 hydrofullereneand/or 1 mM of hydrogen peroxide (H2O2) for 24 hours. This study demonstrated that C60 hydrofullerene and H2O2significantly decreased cell proliferation. The gene expression and conversion of LC3-II/LC3-I were significantly inducedin the separate and combined treatments. The immunofluorescent results observe that H2O2 dramatically inhibits theexpression of GFAP. However, when the cells were treated with both H2O2 and C60 hydrofullerene, GFAP expressionwas significantly restored. The anti-proliferative effects of C60 hydrofullerene and H2O2 are shown in the results. Bothtreatments induced LC3-based autophagy and H2O2 was down regulated GFAP.

} }