TY - JOUR ID - TI - Response of secondary metabolites and some traits of vegetative growth and yield of Brassica oleraceae var. Italica L for spraying with nano-fertilizer and nano-bio-stimulator AU - Mustafa Ibrahim Halim Al-Hamad Abbas K. Mijwel Ban Mohammed Hussain Ali Altaei PY - 2022 VL - 14 IS - 3 SP - 35 EP - 44 JO - Euphrates Journal of Agricultural Science مجلة الفرات للعلوم الزراعية SN - 20723857 AB - A field experiment was conducted during the autumn agricultural season 2020-2021 in Al-Azzawiya area of Al-Mahaweel district, which is located 30 km north of the center of Babylon province, using the RCBD design and arranging Split plot system to study the effect of two factors: the first factor: nano-fertilizer for microelements (zinc 1%, iron 9% and manganese 1%).It was distributed in the main plot with four concentrations (0, 1.5, 1.5, 2) g.L-1 nano fertilizer and the second factor: spraying with nano biostimulator nano-treated seaweed and bacteria extract distributed in the sub plot and at three concentrations (0, 1.2 ml.L- 1 nano biostimulant ) and the results showed: The effect of nano fertilizer positively and significantly at a concentration of 1 g.L-1 on some characteristics of vegetative growth and yield of one plant and gave the highest averages for the studied indicators, the content of leaves of chlorophyll was 77.52 spad, and the leaves area was 7882 cm2 plant-1, plant yield of one was 2,120 kg plant- 1,The treatment of nano-fertilizer significantly affected a concentration of 1.5 g.L-1 and a concentration of 2 g.L-1 in the content of curd of vitamin C, and it reached 151.42 mg per 100 g-1. Also, the treatment gave 1.5 g the highest results in the content of curd of phenols, which amounted to 0.906 mg g-1 dry weight, and the 2 g.L-1 treatment gave the highest results in curd content of flavonoids, which amounted to 37.739 mg gm-1 dry weight, and 1.5 g.L-1 treatment gave the highest results in curd content of peroxidase enzyme, which amounted to 118.61 ml.L- 1, the treatment of 2 g.L-1 gave the highest results in the curd content of catalase enzyme, which amounted to 97.56 ml.L-1 , and the treatment of 2 g.L-1 gave the highest results in the curd content of antioxidants, which amounted to 70.76 mg gm-1 of dry weight, The nano-bio stimulator had no effect on most of the studied vegetative growth traits, except for some traits, including leaves .The biostimulant also gave the best results in the curd content of vitamin C, where the treatment gave 1.5 ml L-1 the best results, amounting to 148.27 mg per 100 g-1, and the treatment gave 1 ml L-1 the best results in the curd content of phenols, amounting to 0.908 mg gm-1 dry weight, The treatment of 1.5 ml L-1 gave the best results in the curd content of flavonoids, which amounted to 37.316 mg g-1 of dry weight, and the treatment of 1 ml L-1 gave the best results in the curd content of peroxidase enzyme, which amounted to 120.34 ml.L-1 . Also, the treatment of 1 ml L-1 gave the best results in the curd content of catalase enzyme , which was 97.23 ml.L-1 .As for the interaction, it had a positive and significant effect on all the studied traits, including the curd content of chlorophyll, where the interaction treatment gave 1 g.L-1 nano fertilizer and 1 ml L-1 nano-stimulator the best treatment and it was 79.29 SPAD.The interaction treatment of 1 g.L-1 nano-fertilizer and 1.5 ml l-1 nano-stimulator gave the best treatment for leaves area, which was 8693 cm plant. The interaction between 1.5 g.L-1 nano-fertilizer and 1.5 ml L-1 gave a higher biostimulant and a percentage in the curd content of vitamin C was 152.33 mg per 100 g-1.The interaction between the spraying with water only treatment and the 1ml L-1 nano biostimulant treatment gave the highest value in curd content of phenols, amounting to 0.931 mg. g-1 dry weight.The interaction between the spraying with water only treatment and the 1.5 ml L-1 nano biostimulant treatment gave the highest value of 38.407 mg g-1 dry weight in the curd content of flavonoids.The interaction between the treatment of 1.5 g.L-1 nano fertilizer and 1 ml L-1 biostimulant gave the highest value and it was 126.44 ml.L-1 in the curd content of peroxidase enzyme. The interaction that treatment 1 ml L-1 nano biostimulant and spraying with water only gave the highest value and reached 99.29 ml in curd content of catalase enzyme, treatment reached 2 g.L-1 and treatment 1.5 ml L-1 the highest value was 72.29 mg g-1 dry weight of the total capacity of antioxidants in curd of broccoli plant.

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