TY - JOUR ID - TI - Potential use of proteolytic bacteria Paenibacillus dendritiformis (BT7) isolated from Batu tannery efuents for the detergent industry AU - Chandran Masi a,b,*, Degafneh Tadesse a, Abate Ayele PY - 2022 VL - 8 IS - 4 SP - 670 EP - 681 JO - Karbala International Journal of Modern Science مجلة كربلاء العالمية للعلوم الحديثة SN - 2405609X 24056103 AB - This research was aimed at identifying a bacterium that can produce alkaline proteases. As a result, bacteria thatproduce proteases were isolated from Batu tannery effluents, tested for protease synthesis on skim milk agar plates, andvalidated with a protease assay. Microscopic and molecular phylogenetic analyses identified Paenibacillus dendritiformis(BT7) as the bacterial isolate with the highest alkaline protease production. The isolate's maximum enzyme productionwas obtained by 2% inoculum size, 40 C temperature, 9.0 pH, and a 48-h incubation time with production mediacomponents such as glucose, casein, MgCl2, and 2% NaCl. The maximal enzyme activity was 270 U/mL under all optimum culture conditions. Concentrated ammonium sulfate precipitation (75%) and dialysis were employed to obtain acell-free, partially purified protease. The specific activity of the dialysate, which accounts for 3% of the enzyme yield, wasdiscovered to be 134 U/mL. The partially purified protease was used for application in blood stain removal. It wasstudied and found that the alkaline protease resistance under stringent conditions is very stable with bleach detergent.Also, this enzyme could clean blood-stained fabrics. This study shows that the alkaline protease from P. dendritiformis -BT7 could be used in various ways in the detergent industry that are good for the environment.

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