TY - JOUR ID - TI - Effects of Breast Milk Treatment on the Growth of Two Transformed Cell Lines تأثير المعاملة بحليب الثدي في نمو نوعين من الخلايا المتحولة AU - Haider S. Abid حيدر صاحب عبد PY - 2010 VL - 3 IS - 2 SP - 1 EP - 6 JO - Iraqi Journal of Cancer and Medical Genetics المجلة العراقية للسرطان والوراثة الطبية SN - 20786123 23135379 AB - Both epidemiological studies and in vitro experiments suggested a potential activity of breast milk as anti-cancerbiological fluid, which provides a promising area that needs more research. In this study, the effects of human wholemilk samples (M1, M2, and M3) taken from three donors on the cell growth of REF and Hep-2 cell lines are tested.The cells were exposed to concentrations of 50, 67 and 75% of each milk sample for 72h. The results demonstratedthat only treatment with M1 50%, M1 67% and M2 75% caused a significant decrease in cell growth of REF cellscompared to the control. Hep-2 cells showed no significant growth response to the concentrations of the human milksamples used. When the results were analyzed concentration-wise regardless of the donor from whom the sample wastaken, 50% milk caused significant growth inhibiting activity for REF cells only, while 75% milk exerted such activityon both REF and Hep-2 cell lines compared to control. When a comparison was made between the responses of the twocell lines to milk treatment, REF cells showed significantly decreased growth percentage compared to Hep-2 cells, butonly with concentration of 50% milk. This study suggests that the cell growth inhibiting activity of whole breast milkis both sample- and concentration-dependent. However, milk samples in the present study might have lost some highmolecularweight biologically active constituents during their preparation to the cell growth assay. The use of pairedtransformed/non-transformed cell lines derived from the same cell type is recommended since such model providesmore comparable results for the effects of human milk on cancer cells.

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