@Article{, title={Evaluation of Stability of Cefamandol and Ceftazidime with Clavulanic Acid Against Extended Spectrum β- Lactamase}, author={Hamoudi A. Hameed and Siham S. Shaokat}, journal={Iraqi Journal of Pharmaceutical Sciences المجلة العراقية للعلوم الصيدلانية}, volume={19}, number={2}, pages={42-47}, year={2010}, abstract={The aim of this study is to evaluate in-vitro activity of Cefamandol (Cfm) and Ceftazidime (Cfz), in combination with Clavulanic acid (CA) against ten complicated multiresistant uropathogenic E.coli .One hundred clinical strains were isolated from patients with chronic urinary tract infections (UTIs), these isolates were identified by the Api identification systems. The antimicrobial susceptibility tests were determined by Kirby-Bauer method, all of them were sensitive to Imipenem (Imp). Ten strains were chosen for the present study, they were resistant to Ampicillin (Amp), Amoxicillin (Amo), Carbenicillin (Cb), Ticarcillin (Tic), Azlocillin (Azl), Amoxicillin Potassium Clavulanate {Augmentin(Amc)}, (AmoCA), Ticarcillin Potassium Clavulanate {Timentin} (TicCA) ,Cefazolin (Cfo) ,Cefaloridin (Cfr), Cefamandol, (Cfm),Cefoxitin, Ceftazidime (Cfz), Cefixime (Cxm), Cefoperazone( Cfp) and Aztreonam (Atm), also resistant to other antibiotics, Tetracycline(Tc),Cloramphenicol(Cm),Gentamycin(G),Amikacin (Amk), Ciprofloxacin (Cip) and Trimethoprim. 50% of the isolates were resistant to Nalidixic acid and Rifampicin. The minimum inhibitory concentrations of Cefamandol and Ceftazidime were determined, by tube method. Transfer of plasmids were done by direct conjugation test to sensitive standard E.coli ,cell free β- lactamases were prepared and detected by macro-iodometric method. The activity of each cell free ß– lactamases extract against Cfm and Cfz were determined by disks diffusion method (microbiological Masuda method). Excellent activities were obtained against these strains when Cfm and Cfz, combined with CA, therefore complete zones of inhibition were obtained indicated the prevalence of extended spectrum β- lactamases in E.coli. The stability of Cfm and Cfz in the presence of CA were useful in the treatment of chronic urinary tract infections caused by multiresistant β- lactamase (ESBL) producer E.coli.

يهدف البحث الى دراسة فعالية السيفامندول (Cfm) والسيفتازديم (Cfz) باضافة حامض الكلافجولانك تجاه ( E-Coli) المقاومة لمجموعة مضادات البيتالاكتام معزولة من مرضى يعانون من التهاب المجاري البولية المزمن وقد بينت الدراسة ان الفعالية المثلى لكل من السيفاماندول والسيفتازديم تكون بوجود حامض الكلافجولانك وان ثباتية كل من السيفاماندول والسيفتازديم بوجود الحامض تشير الى امكانية استخدامهما في معالجة التهاب المجاري البولية الناتجة عن (E-Coli ) المقاومة لمضادات البيتالاكتام من خلال طرائق التقييم والتشخيص التي استخدمت في البحث} }