@Article{, title={Evaluation of Antioxidant Status in Full-term Hyperbilirubinemic Neonates with Severe G6PD Deficiency in Najef: Iraq……………}, author={Dr. Fadhil J. Al-Touma and William M. Frankool}, journal={Karbala Journal of Medicine مجلة كربلاء الطبية}, volume={2 no.6, 7}, number={4}, pages={429-442}, year={2009}, abstract={objective: The objectives of this study were an attempt to evaluate and to comparebetween some of the antioxidant and biochemical parameters investigated in severeG6PD-deficient neonates with hyperbilirubinemia with TSB≥ 15 mg/dl in Najefgovernorate : Iraq.Methods: A total of 240 neonates who were admitted in Al-Zahra maternity and pediatricshospitals in Najef governorate during 1st , Oct., 2007 and 12th, July, 2008 with age rangedbetween 1 – 28 days were screened for erythrocyte G6PD enzyme activity measurement toconfirm the diagnosis of G6PD deficiency. Of these subjects, 61 (25.42%) neonates of themshowed a normal enzyme activity levels ; whereas the remaining 179 (74.58%) neonates werefound to have neonatal hyperbilirubinemia with TSB levels ≥ 15 mg/dl. Some of the antioxidantparameters have been measured in severe G6PD-deficient neonates with hyperbilirubinemia andcompared with that found in control group.Results: Among the hyperbilirubinemic neonates, only 21 (11.73%) neonates were diagnosed tohave severe G6PD deficiency and its percentages of incidence identified was 11.73%. The resultsalso indicated that there was a significant negative correlation (r = - 0.320 , P < 0.05) between thedecreased G6PD activity levels and the elevated TSB concentrations in severe G6PD-deficienthyperbilirubinemic neonates with the TSB ≥ 15 mg/dl but not in control neonates. These datasuggest that the G6PD-deficient neonates are at increased risk for hyperbilirubinemia even in thenursery free from agents that can potentially cause hemolysis to G6PD-deficient red cells. Themean ± SD of oxidative stress status parameters which include erythrocyte GSH, MDA, G-Red,G-Px and catalase were determined. There was a significant decrease in each of erythrocyte GSH,G-Red and catalase activity levels (P<0.05), whereas the lipid peroxidation end product MDAlevels and G-Px activity levels were significantly increased in all hyperbilirubinemic neonates (P< 0.05) as compared with the control group. G6PD activity values identified were found to bepositively correlated with each of GSH concentrations, G-Red and catalase activity levels inwhich their values were found to decreased in patient groups, while it was found to be negativelycorrelated with each of G-Px activity and MDA levels in which their values were elevated insevere G6PD-deficient neonates. These data indicates an increases in free radical generation andthus antioxidant defense mechanisms is impaired in peroxidation associated with a significantelevation in MDA levels in the erythrocytes of the hyperbilirubinemic neonates with severe G6PDdeficiency than that found in the control group which demonstrate the presence of an increasedoxidative stress due to reduction in NADPH which is generated in RBCs by HMP-shunt only.Conclusion: The results of the present work indicate that severe deficiency of G6PD activity isassociated with hyperbilirubinemia in full-term neonates and imbalance in oxidative stressparameters..

} }