TY - JOUR ID - TI - Immunohistochemical study of BCL-2, PCNA and VIMENTIN markers in oral and laryngeal squamous cell carcinoma (Comparative study) AU - Shaimaa F. Muhsin شيماء محسن AU - Kadhim A. Alsodani كاظم السوداني PY - 2011 VL - 23 IS - special issue SP - 82 EP - 86 JO - Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد SN - 18171869 23115270 AB - Background: Squamous cell carcinoma (SCC) may arise in any stratified squamous epithelium or muosa that hasundergone squamous metaplasia. The aims of the study were immunohistochemical evaluation and comparison ofPCNA, BCL-2 and VIMENTIN expressions in oral and laryngeal squamous cell carcinoma and correlating suchexpressions with the clinicopathological behavior in both sites.Materials and Methods: This study was performed on thirty formalin-fixed, paraffin-embedded pathologicallydiagnosed oral and laryngeal SCC blocks for the period of June 2006 till July 2010.Age, sex, site and histologic gradeswere recognized. The samples were immunohistochemically stained with monoclonal antibodies toBCL-2, PCNA andVIMENTIN.Results: The age of the patients with squamous cell carcinoma of the oral cavity was between 40 and 86 andbetween 32 and 66 years in larynx. The male/female ratio was 10/5 and 8/7 for oral and Larynx respectively. Themajority of the cases of Oral squamous cell carcinoma were located on the buccal mucosa, Whereas Laryngealsquamous cell carcinoma cases were distributed on glottic mainly..No significant statistical difference in the age andgender between the two groups .Histological grading was recognized for each case of oral and laryngeal squamouscell carcinoma. Two thirds of the oral cases were well differentiated. While 8 of the fifteen cases were moderatelydifferentiated SCC. Regarding histological grading there was no statistically significant difference between the twogroups .PCNA expressed in 14 of the fifteen oral cases whereas in laryngeal, 12 cases were positive. Collectively, 9 ofthe 30 cases were positive for vimentin antibody with different score values,.Bcl-2 was expressed in one case onlyfrom fifteen immunostaining laryngeal cases and all the oral squamous cell carcinoma cases were negative Nosignificant relationship in immunoexpression of he above markers between the tow groups.Conclusions: The results of this study proved that the biological behavior namely the PCNA, VIMENTIN and BCL-2activities was comparable between SCC of the oral cavity and larynx with a spectrum of clinical behavior, due tothe differences in location

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