TY - JOUR ID - TI - Detection of genomic instability in oral squamous cell carcinoma using random amplified polymorphic DNA based on polymerase chain reaction method (RAPD-PCR) AU - Shawki Shanan Abed شوقي شنان عبد AU - Nadia S. Yass نادية ياس AU - Majeed A. Sabah مجيد صباح PY - 2012 VL - 24 IS - special issue 2 SP - 81 EP - 83 JO - Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد SN - 18171869 23115270 AB - Background: Oral squamous cell carcinoma is an invasive epithelial neoplasm, occurred most commonly in alcoholicand tobacco using adults. The present study is aimed to identify the genomic instability in OSCC patients usingrandom amplified polymorphic DNA (RAPD), a polymerase chain reaction (PCR) based technique.Materials and methods: Twenty five blocks of formalin fixed paraffin embedded tissue was used as malignant DNAsource and five sample of healthy DNA obtained from the oral tissue and blood. Using DNA extraction kit (Geneaidminikit) and eleven random sequencing primers to visualize the amplifications pattern under UV.Results: The primer detectability of genomic instability ranged from 21% in well differentiated OSCC to 68% in poorlydifferentiated OSCC. CasesT8 and T13 showed highest genomic instability (75%). The results determined numbers ofgenomic instabilities among OSCC patients by comparing the pattern of amplifications of the primers in bothmalignant and healthy DNA.Conclusions: High significance correlation between primers detection rate and histopathological grade of OSCC.Further larger studies are needed to: 1) Obtain RAPD markers useful for OSCC for early diagnosis; 2) investigatedifferent genes directly involved in the etiology of OSCC; 3) analyze chromosomal instability among OSCC patients

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