@Article{, title={Expression of RANKL by dental cells during eruption of mice teeth}, author={Lubna K. Jassim لبنى جاسم and Athraa Y. Al- Hijazi عذراء يحيى الحجازي}, journal={Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد}, volume={25}, number={1}, pages={76-81}, year={2013}, abstract={Background : In order for a tooth to erupt, two obvious requirements are needed. First, there has to be alveolar boneresorption of the bone overlying the crown of the tooth such that an eruption pathway is formed. Second, resorptionof bony crypt and apposition of new one, third, there has to be a biological process that will result in the toothmoving through this eruption pathway.The amniotic sac contains a considerable quantity of stem cells. Theseamniotic stem cells are multipotent and able to differentiate into various tissues, which may be useful for humanapplication. Receptor activator of nuclear factor kappa B ligand (RANKL) is concentrated on bone biology, morespecifically bone metabolism. RANKL plays a vital role in osteoclastogenesis for bone resorption. This study aimed toevaluate the expression of RANKL marker by dental cells during eruption of the teeth.Materials and Methods: : forty eight albino Swiss mice of one day old age injected with isolated amniotic stem cellsin the anterior region of maxilla (incisors area) other 16 mice injected with saline represents control. Sacrifice 4 micefor each period (4, 7, 10, and 13) day old age. The result were studied histologically and immunohistochemistry.Results: The present results localized and identified RANKL marker in 3 areas of developing tooth of the studied groupsincludes overlying, surrounding and apical bone. Positive RANKL with high significant value expressed by osteoclastof overlying bone in Amnion group followed by Control at day 4. In surrounding bone positive expression of RANKLillustrated to be highest in Control followed by Amniotic fluid at day 10.Apical bone shows positive expression ofRANKL in amniotic fluid group and it records to be the highest value in comparison to studied groups at day 10.Conclusion Expression marker RANKL illustrates that amniotic fluid group has a high expression of RANKL in osteoclastsurrounding and apical bone areas while control expressed RANKL in osteoclast of overlying bone. The present resultsopened clinical hopes in dental tissue engineering by application of autologous amniotic fluid and chorion cells

} }