TY - JOUR ID - TI - Oxidative Stress and Antioxidant Defense in Patients With Jaundice أجهاد التأكسد و الدفاع المضاد للتأكسد في مرضى اليرقان AU - 1Suhayla Khalied Mohammed سهيلة خالد محمد1 AU - 2Perry Habib Saifullah و بيري حبيب صالح2 AU - 3 Hazzim Hussein Edan حازم حسين عيدان3 AU - 4Numan Abd Allateef نعمان عبد اللطيف4 PY - 2012 VL - IS - 1 SP - 40 EP - 60 JO - AL-yarmouk Journal مجلة اليرموك SN - 20752954 AB - Background: The objective of our study is to measure serum Malondialdehyde level (MDA), an index of oxidative stress, and Vitamin E level, a protective agent against oxidative stress, in Jaundice patients and study the possible correlation between them . Materials and Methods: 80 patients with jaundice ( 38 female and 42 male) and 50 healthy control (23 female and 27 male) were enrolled in this study. About 10 ml of venous blood were obtained from the cubital vein using disposable needles and syringes. The thiobarbituric acid method was used to measure the Malondialdehyde (MDA) which reacted with thiobarbituric acid(TBA) to give pink color that was read at (535 nm). The concentration of vitamin E in serum was determined according to a modified Hashim and Schuttringer method. Results: A significant increase (P<0.0001) in serum Malondialdehyde (MDA) in jaundice patients as compared to that of normal healthy controls was observed. A significant decrease (P<0.0001) in serum vitamin E in jaundice patients as compared to that of normal healthy controls has been noticed. No significant correlation between serum Malondialdehyde (MDA) and serum Vitamin E in jaundice patients was observed. Conclusion: our data shows that antioxidant capacity decreases with an increase in oxidative stress in Jaundice patients but there is no significant correlation between them . Abbreviations: The abbreviations used are: dL, deciliter ; MDA, Malondialdehyde ; μl, microliter; r, Correlation factor ; rpm, Round per minute; TCA, Trichloroaceticacid ; TBA, thiobarbituric acid ; ROS, Reactive oxygen species.

يهدف هذا البحث الى قياس مستوى مالون ثنائي الالديهايد(MDA), كمؤشر الى الاجهاد التأكسدي, و مستوى فيتامين E,و هو عامل وقائي ضد الاجهاد التاكسدي , في مصول مرضى اليرقان وتقصي امكانية وجود ارتباط بينهما ER -