TY - JOUR ID - TI - Inhibition of bacterial growth around gutta percha cones by different antimicrobial solutions using antibiotic sensitivity test (An in vitro study) AU - Nagham A. Al-Hyali نغم الحيالي PY - 2013 VL - 25 IS - 4 SP - 26 EP - 32 JO - Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد SN - 18171869 23115270 AB - Background: Decontamination of gutta percha cones was important factor for success of root canal treatment. Theaim of the present in vitro study was to identify and to compare the antimicrobial effect of following disinfectionsolutions: 0.2% chlorhexidine gluconate, Iodine, tetracycline hydrochloride solution, EDTA & formocresol mixed withzinc oxide eugenol, on E faecalis, E coli and Candida albicans using sensitivity testMaterials and Methods: Three types of microorganisms were isolated from infected root canals (E faecalis, E coli andCandida albicans) and cultured on Mueller Hinton agar petri-dishes. Disinfection of gutta percha cones done byimmersion in six disinfection solutions (six groups), the groups are: distill water (used as control group), 0.2%chlorhexidine gluconate, Iodine, tetracycline hydrochloride solution, EDTA & formocresol mixed with Zinc oxideeugenol. The immersion times were 5 min. and the incubation was aerobically at 37 °C for 24 hr. for bacterial speciesand at 28°C for 48 hr. for candida albicans. After incubation, zones of inhibition (no growth of bacteria) wereexamined around the gutta percha cones & diameters of the zones were measured with a boley gauge. The meansof inhibition zones for each group were measures and statistically analyzed among groups using ANOVA and LSDtests at 0.05 significance level.Results: There is highly significant differences (P=0.000) among all the tested groups. 0.2% Chlorhexidine gluconateshowed the maximum antibacterial efficacy (broader zones of inhibition) against E faecalis. Compound offormocresol + Zinc oxide eugenol showed the maximum antibacterial efficacy against Candida albicans, and E coli.The minimum antibacterial efficacy against Candida albicans occurred with tetracycline group, while against Efaecalis and E coli occurred with EDTA groupConclusion: All the tested materials had antibacterial efficacy against Candida albicans, E faecalis and E coli; butchlorhexidine gluconate and compound of formocresol & Zinc oxide eugenol, are more effective agents for a rapiddisinfection of gutta-percha cones in five minutes

ER -