@Article{, title={Evaluation of Antibacterial Efficacy Against Enterococcus Faecalis of Newly Prepared Endodontic Irrigant Solution (an In vitro study)}, author={Nawfal A.A. Zakarea and Talal Hameed Mohamad and Amer A Taqa}, journal={Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان}, volume={14}, number={1}, pages={153-160}, year={2014}, abstract={To evaluate the efficacy of a newly prepared endodontic irrigant solution, against E. faecalis.Materials and Methods: Sixty human extracted single rooted teeth samples were prepared by usingprotaper NiTi rotary system. The apical foramen of each sample was sealed by acrylic resin on theapical 3mm. Each root was embedded in silicon impression material block and autoclaved. A ten μl(4X10P5P) of inoculated broth media (nutrient broth) with E. faecalis was injected gently inside thecanals of group A,B&C was incubated for 24hours at 37CPoP. Group D was left without inoculation toserve as a control negative. The samples of group A,B and C were disinfected as fallow: Group A wasdisinfected with MCP solution(mixture of 20% castor detergent and 4% papain). Group B wasdisinfected with freshly diluted 2.5% NaOCl solution. Group C not treated with any solution controlpositive. The disinfected solutions were left inside the canal for 5 min then each canal was dried withsterilized paper point, then bacterial samples were taken from the samples of 4 groups using size 40sterilized file which was inserted to full canal length and rotated 360 degrees in clock wise direction fordentin engagement. The file was put inside the sterilized plastic tube containing 1ml of normal salinethen 200 μl was inoculated and spread on Enterococcus agar media and incubated at 37CPoP for 18hours,the numbers of bacterial colonies were counted. Results: Both solutions (MCP and NaOCl) producecomplete eradication of E Faecalis within 5 minutes. Conclusion: Castor detergent 20% and papainenzyme 4% (MCP) has ability to completely eradicate Enterococcus Faecalis bacteria from theinfected root canal in vitro in 5 min. It's antibacterial action is similar to the action of 2.5 % NaOCl

} }