دراسة مناعية لتقييم كفاءة لقاح مستضد متعدد السكريد لجدار خلية الفطرAspergillus niger في حيوانات ذكور الارانب

Abstract

This current study aimed to evaluate the efficiency of polysaccharide antigen vaccine which had been isolated from wall of A.niger fungi isolated from TB pateints sputum and soil, in order to provide future protection against infection with higher concentration of A.niger conidia. Six New Zalent white male rabbit had been immunoized with antigen in addition to another group (of 6 rabbit) as control by used the fungi conidis . Challenge with conidia were occurred on both groups by IV A morphological and behavioral changes appeared with control animals treated with challenge dose while the immunized one have no change but the size of spleen and lung in control animals injected with challenge dose increased the spleen weight 14.04±2.13 and 11.51±3.56 the lung weight for animals Immunized with A.niger conidia which isolated from soil and sputum of Tuberculosis patients(TB). While for animals immunized with A.niger polysaccharide antigen and treated with challenge dose of A.niger conidia from TB patients the spleen weight 10.89±3.23 and lung weight 18.58±3.96 .Compared to that immunized with A.niger polysaccharides antigen from soil and treated with challenge dose of A.niger conidia from soil where spleen weight 10.39±0.99 and lung weight 13.25±3.99.However, the histological cuture of these organs (lung& spleen) on sabouraud Dextrose agar had been done in order to indicate the ability of body to eradicate fungi conidia and ability to prevent infection. These result indicate presence of A.niger colony for spleen and lung culture of control animals (6 rabbit) treated with A.niger conidia isolated from soil and pateints with TB. While the absence of such colony in culture of lung and spleen of immunized animals