Effect of crude extract of Origanum vulgare on the inhibition of some pathogenic bacteria and causing spoilage of food

Abstract

Aromatic and medicinal plants are wide spread throughout world this study was conducted to investigate the inhibitory role of crude extract of Origanum vulgare plant on some pathogenic bacterial species :Pseudomonous aerogenosa, Staphylococcus aureus, Salmonella typhimurium, E. coli, Listeria monocytogenus, Streptococcus pyogenus, Brucella abortus and Bacillus anthracis. These strains were cultured on nutrient agar then each Petri dish contain one of the pathogenic bacteria has been injected into the three holes by extract concentration 50 mg/ml and 100 mg/ml) and ethanol alcohol 70% as control, the inhibitory zone were measured. The results reaveld that the inhibitory effect of alcoholic extract was more effective against Listeria monocytogenus in inhibition zone diameter (31-32 mm), followed by Staphylococcus aureus in inhibition zone diameter (27-32 mm) then Salmonella typhimurium, E. coli and Brucella abortus in inhibition zone diameter (26-30 mm, 25-29 mm, 25-30 mm) respectively and moderate activity on Streptococcus pyogenus, Pseudomonous aerogenosa in inhibition zone diameter (19-20 mm, 19-28 mm) then Bacillus anthracis in inhibition zone diameter (17-25 mm) respectively