Detection of Carbapenem-Resistant Klebsiella pneumonia using two Sensitivity Methods

Abstract

Carbapenems, potent β-lactams antibiotics, were initially used as last-resort treatmentsfor severe Gram-negative bacteria such as Klebsiella pneumonia. These bacteria wereconsidered one of Enterbacteriaceae family associated with pneumonia and urinary tractinfection. In the current research, Carbapenems were studied to identify the resistant K.pneumonia isolates and identifying the relation with some β-lactam resistance to avoid theirabuse for treatment.A urine sample got from 80 infected female with chronic urinary tract infection, thesesamples tested for K. pneumonia and their carbapenemase production. The procedure wasbased on disc diffusion and Landman e,t al. methods.The results pointed out that Klebsiella pneumonia is significantly present in patientswith urinary tract infection, the carbapenem resistance in disc diffusion and Landmanprocedure was 100% and 88.5% for meropenem (MEM), while it was 57.2% and 65.4% forimipenem (IPE), respectively. Regarding the carbapenem resistance in same isolate for both(MEM and IPE), 69.23% (18/26) were coincidence and 30.77% (8/26) were mismatch in bothmethods. In addition, the Landman procedure more accurate than disc diffusion forcarbapenem resistance and there is a strong association with Beta-lactam resistance.It can be conclude that there is a high incidence of carbapenem resistance in localisolates of Klebsiella pneumonia. We recommend to use the supercarba media in diagnoses ofbacteria routine work because it is more specific along with disc diffusion method for patientswith urinary tract infection and the use of other treatments to decrease the level of resistance