Determination the inhibitory efficiency of hybrid nano chlorhexidine against some gram negative bacteria in vitro

Abstract

The inhibitory activity of each of the nano disinfectant Chlorhexidine] Magnisum- Alaminum-Chlorhexidine-Layered Double Hydroxide (Mg-Al-CHX-LDH)[ in addition to free Chlorhexidine (CHX) were assessed by diffusion agar method against Pseudomonas spp. , Klebsiella pneumonia and Acinetobacter baumannii. Results showed that highest inhibitory activity was obtained against isolates K. pneumonia 16-B and 27 with average inhibition zone of 15.25 and 15.16 mm , respectively, while isolates P. aeruginosa 4-C and 15-A were the lowest affected with average inhibition zone of 6.87 and 6.75 mm, respectively. The antimicrobial susceptibilities test was carried out for 15 antibiotics and the susceptibility of the bacteria to different antibiotics varied depending on the isolate, Ciprofloxacin and Netilmicin were the most effective antimicrobial agents against K. pneumonia and A. baumannii , respectively . Generally, high resistance among the tested isolates was detected.