تأثير الماء الممغنط في الأجهاد المائي من خلال أستحثاث كالس نبات الحلبة Trigonella foenum graesum L.

Abstract

This study aimed to induce callus form Trigonella foenum graecum L. in vitro on MS medium after supplying it with different combinations of auxin 2,4-D cytokinin BA. The results showed that BA 1mg/L +2,4-D 1mg/L was the best combination which gave the highest callus compared to other treatments. The effect of adding water stress (PEG) to medium in different concentrations (0,3, 6, 9%) and adding water stress with magnetic water (%3+1000G.,%6+1000G.,%9+1000G.) were the best results, which gave the highest weight is (%3 and %3+1000G.). Effect of PEG in the media led to a decrease in fresh and dry weight of callus for all samples except at %3 which was showed significant increase in the weight. The addition of PEG and magnetic water on the media cause reduction in fresh weights except the concentration of %3+1000 G. which gave increasing in fresh weight. the magnetic water was kept the dry weight