The effect of some biological and chemical treatments in the control of the growth of one of pathogenic fungi isolated from the seeds and roots of the tomato plant

Abstract

This study included testing the efficacy of two fungal filtrates of Trichodermaharzianum and Trichoderma aureoviride and the chemical compounds of ammoniumchloride and calcium chloride in the radial growth and germination of spores of thepathogenic fungus Fusarium oxysporum isolated from the seeds and roots tomato inmeasuring with fungicide (Topsin), as well as test the effect of these treatments on thegermination of the seeds of tomato in sterilized and unsterilized soil,It also has beendiagnosed pathogenic fungus F. oxysporum using a technique Polymerase Chain Reaction(PCR).The results showed that all treatments salts and filtrates have a significant inhibitoryeffect to the growth for the fungus F. oxysporum on the solid culture medium P.D.A inmeasuring with controlled treatment at the level possibility 5%,as it was for these treatmentshave a significant inhibitory effect to the germination of spores for the tested fungus, as wellas the back of a clear effect in raising the percentage of germination of the seeds of tomato insterilized and un sterilized soil, and It was the concentration 15% of the filtrates and 15 mg/mlof salts most influential in measuring with other concentrations and fungicide (Topsin) atconcentration of 1mg/ml .