Detection of Entamoeba histolytica in patients an infected infants with diarrhea in born and children's hospital by classic methods and Real-timePolymerase Chain Reaction.

Abstract

The results of this study were including detection of Entamoeba histolytica in an in patients infected infants with diarrhea in born and childrenʾs hospital in Al-Diwania province during the period from June until December 2014, the number of samples was 170 stool sample for age between one month-two years, these samples were examined by direct smear method by using physiological normal saline and Iodine solution, there 88 samples were positive in percentage %51.76 also the lower percentage was %36.17 recorded in age 1-6 months and the higher percentage was %61.76 in age (1-6)-two years, wherever the percentage of an infected male was closely which reach %53.16 ,%50.55 respectively. According to type of feeding, the higher percentage was %59.79 in an infants with Artificial feeding compare with the percentage %41.09 in an infants with breast feeding, wherever the percentage of infection with parasite was %57.32 in rural region ,its higher than the percentage %59.46 in urban area, also the results were showed the percentage of infection with this parasite was %44.32 by using Real-time polymerase chain reaction as confirmative test .