Determination of Total Fatty Acids and Natural Antioxidants in Rice Bran Oil and Study there Nutritional Effects

Abstract

This study was conducted in the Agricultural Directorate labs of Kirkuk . It included the use of Mchkab 2 Class Rice Bran (RB) which produced from El-Ameer mill factory in Najaf province at 2014 , and then thermally treated at 120 C for 10 minutes to inhibited the lipeases. Estimation of the total fatty acid using G.C Showed that (RBO) has a rate of 17.59% Saturated fatty acids and 80.09 % unsaturated fatty acids , the results that Mono Unsaturated Fatty Acids (MUFA) 41.67% and 38.42% Poly Unsaturated Fatty Acids (PUFA). Antioxidants estimation by using High Performance liquid Chromatography (HPLC) Showed that the content of (RBO) of each oryzanol and tocopherols and tocotrinols was 6.1 and 0.564 and 0.822 mg/g ,respectively. The results of Bio experiment to study the effect of diet subsidized by 2.5% of (RBO) of fat in the blood of female rats showed a significant decrease (P≤0.05) in the value of each T.C , T.G , LDL and VLDL 123.19 , 99.13 , 51.06 and 19.82 mg/dl respectively, with noticing a significant increase (P≤0.05) in the value of HDL at a level wich has reached 52.31 mg/dl after it was 48.43 mg/dl for the control treatment (female rats fed on normal diet).