Preparation of live attenuated vaccine of Streptococcus equi equi by submucosal injection in the upper lips of rabbits.

Abstract

Preparation of live attenuated vaccine of Streptococcus equi equi by submucosal injection in the upper lips of rabbits.A.M.H.Al- Judi M.A.SalihAbstractLive attenuated vaccine of Strept. equi equi was prepared by culturing the bacteria in increasing concentration of acriflavin beginning from 2ppm to 16ppm by 77 passage for three months.The first group recived submucosal injection in the upper lip , and the second group recived intranasal installation of the vaccine. The titer of antibodies in the first group was 853.33 ± 107.93 8 weeks post vaccination, & 384 ± 57.24 in the second group with significant difference at the level of P< 0.05 between the two groups. Both groups did not show any clinical findings after challenge dose , while the control group died.