Effect of diluents in prevention of virus inactivation during drinking water vaccination against Newcastle disease

Abstract

Effect of diluents in prevention of virus inactivation during drinking water vaccination against Newcastle diseaseA.A.S.AL-Mayah H. A. ALTaher Q. A. AL-Obodi N. A.Shehan D.H.Sadek. E.A.HColl. of Vet. Med./ Unive of BasrahAbstractNewcastle disease (ND) drinking water type vaccines secured at the time of actual field application and adequate viable virus were proven to produce an immune response .ND virus vaccines of live lentogenic strains including Hitchner B1 and LaSota were used in this study. These two vaccines were given to the birds of all groups at 7th and 21st day of age respectively. The vaccines were thoroughly mixed with distilled water and administered to group A. Group B was administered the vaccines with Reverse Osmosis (R.O.) water, whereas group C was given the vaccines after mixing with tap water. Tap water plus powder skim milk (P.S.M.) as a diluent for vaccines was given to group D, while group E was served as negative control. Each group was consisted of 20 birds .Blood samples have been collected randomly from 10 chicks of each group 14 days post last vaccination and the serum was stored in deep freezing until needed. The mean hemagglutination inhibition (HI) titer of each group indicated that the addition of P.S.M.at 1:400w/v concentration overcame the adverse effects of tap water resulting in titer which was higher than that of tap water only. The results also indicated that chicks vaccinated with ND vaccines when prepared in R.O.drinking water vaccination showed a high HI titer as compared with that chicks vaccinated with ND vaccines prepared in tap water only. When distilled water, tap water + P.S.M. and R.O. were used in drinking vaccination against ND, HI tests showed that all chicks were given a high immune response to ND vaccine and only chicks vaccinated with ND vaccine prepared in the tap water had given a low immune response to ND vaccine. The results of this study indicated the great value of R.O. in drinking water vaccination of chickens.